goat anti human plexind1 affinity purified abs  (R&D Systems)

Journal: Cell reports

Article Title: Semaphorin3A/PlexinA3 association with the Scribble scaffold for cGMP increase is required for apical dendrite development

doi: 10.1016/j.celrep.2022.110483

Figure Lengend Snippet: (A and D) Representative images of rat CA1 pyramidal neurons, P7, transfected in utero at E17.5 with shRNA for PlexinA3 (shPlexinA3), NP-1 (shNP-1), PlexinA4 (shPlexinA4), or PlexinD1 (shPlexinD1), or LRR, or control vector (A). Neurons co-transfected with WT-PlexinA3, PlexinA3-ΔC2 (associated Scribble; ), or PlexinA3-ΔCT (did not associate Scribble; ), or functional sGC (co-expressing sGC-α1 and -β1 subunits) (D). Neurons also co-transfected with shPlexinA3 and LRR (shPlexinA3 + LRR) (D). Neurons co-transfected with dTom marker. SO, SP, and SR layers as in . Scale bar, 100 μm. Bottom: sample tracings of neuritic arbor of representative neurons. Scale bar, 20 μm (see and ). (B and E) Quantification of average total apical dendrite length per cell for CA1 pyramidal neurons transfected as in (A) and (D) (n = 20–52 cells; data for control, shPlexinA3, and LRR were pooled among B and E). Control versus LRR or shPlexinA3 + LRR, one-way ANOVA, Dunnett’s multiple comparison test: ***p ≤*** 0.001; all other conditions, one-way ANOVA, Tukey’s multiple comparison test: ***p ≤ 0.001; ****p ≤ 0.0001. Data for shPlexinA3, shPlexinA4, and shPlexinD1 were not significantly different (ns) from control. (C and F) Quantification of average total apical dendrite branch points per cell; same dataset as in (B) and (E). Data for Control, shPlexinA3, and LRR were pooled among (C) and (F). Control versus shPlexinA3, LRR, or shPlexinA3 + LRR, one-way ANOVA, Dunnett’s multiple comparison test: **p ≤ 0.01; ***p ≤ 0.001; all other conditions, one-way ANOVA, Tukey’s multiple comparison test: **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001; shPlexinA3 versus shPlexinA3 + WT-PlexinA3, Student’s t test, **p ≤ 0.01; data for shPlexinA4 or shPlexinD1 were not significantly different (ns) from control. **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001. Error bars represent SEM.

Article Snippet: goat anti PlexinD1 , Santa Cruz , Cat#sc-46245 (E-13).

Techniques: Transfection, In Utero, shRNA, Plasmid Preparation, Functional Assay, Expressing, Marker

Journal: Cell reports

Article Title: Semaphorin3A/PlexinA3 association with the Scribble scaffold for cGMP increase is required for apical dendrite development

doi: 10.1016/j.celrep.2022.110483

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: goat anti PlexinD1 , Santa Cruz , Cat#sc-46245 (E-13).

Techniques: Recombinant, Western Blot, Plasmid Preparation, Software